Anura Hewagama, Ph.D.
4308 Hillside Dr.·Ann Arbor, MI 48105·Cell Phone: (734) 678-0724·Email: [email protected]
CITIZENSHIP: USA
CERTIFICATION: Michigan Secondary Teacher Certificate in Chemistry (DC).
EDUCATION
Mater of Arts in Teaching (2ry Chemistry): Eastern Michigan University, MI 06/2014-02/2015
Doctor of Philosophy, Biochemistry and Molecular Biology: Wayne State University, Detroit, MI
09/1992-08/1999
· Minor Subjects in Pharmaceutical Science and Computer Science
· Awards: Graduate Travel Award 1999, American Society for Biochemistry and Molecular Biology
Master of Science, Chemistry: Eastern Michigan University, Ypsilanti, MI 09/1990-06/1992
Post Baccalaureate Certificate, Molecular Laboratory Diagnostics: Michigan State University, Lansing, MI. GPA 4.0 06/2005-06/2006
Bachelor of Science, Biology/ Chemistry: University of Sri Jayewardenepura, Sri Lanka. 03/1982-05/1986
· Second Class Honors
TEACHING EXPERIENCE
Teacher – Chemistry: East English Village Preparatory Academy, Detroit Public Schools Community District, Detroit, MI 02/2016-Present
Adjunct Instructor –Chemistry: Lansing Community College, Lansing, MI 08/2016-Present
Teaching Internship: Lincoln Consolidated High School/Eastern Michigan University, Ypsilanti, MI
01/2015-06/2015
Graduate Teaching Assistant: Department of Biochemistry and Molecular Biology, Wayne State
University, Detroit, MI 09/1993-05/1994
Graduate Teaching Assistant: Department of Chemistry, Eastern Michigan University, Ypsilanti, MI
09/1990-05/1992
Instructor: Chemistry/Botany, University of Sri Jayewardenepura, Sri Lanka 06/1986-06/1989
PROFESSIONAL EXPERIENCE
Research Investigator: Rheumatology, University of Michigan, Ann Arbor 11/2009-12/ 2012
· Characterized molecular markers in Lupus disease development
· Received pilot funding from Michigan Institute for Clinical & Health Research (MICHR)
· Publications: PMID:22142890, PMID:23434382 and PMID:23576011
Research Lab Specialist Sr.: Rheumatology, University of Michigan, Ann Arbor 10/2006-11/2009
· Identified novel genes and biological pathways with potential contribution to lupus pathogenesis
· Publications: PMID: 19279650,PMID: 19349147 and PMID: 19675166
Research Lab Specialist Sr./Lab Manager: Human Genetics/Bioinformatics,
University of Michigan, Ann Arbor 07/2002-10/2006
· Regulation of gene expression and systems biology - Verification of microarray data using real time PCR, quantitative chromatin immunoprecipitation experiments to analyze NFkappaB regulated genes and pathways.
· Other responsibilities included training, technical assistance and supervision of new lab personnel and the laboratory management.
Post Doctoral Research Fellow: Roche Diagnostics, Indianapolis, IN. 08/2001-07/2002
Advisor: Armen B. Shanafelt, Ph.D.
· Antibody engineering – Structure guided design of antibodies for optimized affinity and specificity to antigens. Our research team solved the structures of the cocaine and morphine bound antibodies.
· Publications: PMID: 15885702 and PMID: 15019787
Postdoctoral Research Fellow: Department Biochemistry and Molecular Biology, Wayne State University, Detroit, MI. 12/1999-08/2001
Advisor: David Evans, Ph.D.
· Understanding the regulation of the mammalian pyrimidine biosynthetic pathway by kinase-mediated phosphorylation of the multifunctional protein CAD. Developed and improved HPLC assay conditions in monitoring the nucleotide levels during the regulation process.
Graduate Research Assistant: Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, MI 09/1994-06/1999
· Doctoral Thesis Research - Studied the mechanism of glutamine hydrolysis and the interdomain interactions of mammalian carbamoyl phosphate synthetase. By using steady state and pre-steady state kinetic studies, site-directed mutagenesis, and homology modeling approaches, we elucidated the molecular mechanism of glutamine hydrolysis and the regulation of the coupled synthesis of carbamoyl phosphate synthetase.
· Publications: PMID: 10497179,PMID: 9858783
Student Training in Research
· Participated in the University of Michigan Undergraduate Research Opportunity (UROP) program and supervised and trained 6 students 09/2009-12/2012
· I have also trained and supervised several work study students and high school students in laboratory research
Current Teaching Interests
· General and advanced Biochemistry
· Introductory level general, inorganic, and organic chemistry
· Application of Bioinformatics tools in Data mining and Biological Pathway Analysis
· Biotechnology and Life Science Research
COMMUNITY/ADMINSTRATIVE
MHealthy Wellness Champion (2010-2012)- University of Michigan
· Promoted and encouraged MHealthy programs and activities among colleagues and U of M Community.
Sports
· Cricket - Represented and captained the Ann Arbor Lions Cricket Club (2012-2015).
· Badminton - Represented and captained University of Sri Jayewardenepura at the inter-college badminton meets. I was awarded the University Badminton Colors (1982/83, 1983/84 and 1984/85 academic years). Currently a member of Ann Arbor Badminton Club.
Invited Reviews
Arthritis Research & Therapy – June 2012
Rheumatology Current Research- 2012 - 2014
Invited Editorial Board Member
OMICS Publishing Group- Rheumatology: Current Research-Open Access – 2012- 2014
Memberships in Professional Societies
January 2016 – Present Member, The National Society for Leadership and Success
March 2015 – Present Member, American Chemical Society
February 2015-March 2016 Member, Association for Education Communication and Technology
1996-2012 Member, American Society for Biochemistry and Molecular Biology
1996-2001 Member, Biophysical Society
PUBLICATIONS IN PEER-REVIEWED JOURNALS
1.Hewagama A, Guy HI, Chaparian M, Evans DR. (1998). The function of Glu338 in the catalytic triad of the carbamoyl phosphate synthetase amidotransferase domain. Biochim Biophys Acta. 1998 Nov 10; 1388(2):489-99. PMID: 9858783
2.Hewagama A, Guy HI, Vickrey JF, Evans DR. (1999). Functional linkage between the glutaminase and synthetase domains of carbamoyl-phosphate synthetase. Role of serine 44 in carbamoyl-phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD). J Biol Chem. 1999 Oct 1; 274(40):28240-5. PMID: 10497179
3.Pozharski E, Wilson MA, Hewagama A, Shanafelt AB, Petsko G, Ringe D. (2004). Anchoring a cationic ligand: the structure of the Fab fragment of the anti-morphine antibody 9B1 and its complex with morphine. J Mol Biol. 2004 Mar 26; 337(3):691-7. PMID: 15019787
4.Pozharski E, Moulin A, Hewagama A, Shanafelt AB, Petsko GA, Ringe D. (2005). Diversity in hapten recognition: structural study of an anti-cocaine antibody M82G2. J Mol Biol. 2005 Jun 10; 349(3):570-82. PMID: 1588502
5. Hewagama A, Patel D, Yarlagadda S, Strickland FM, Richardson BC. (2009). Stronger inflammatory/cytotoxic T cell response in women identified by microarray analysis. Genes Immun. 2009 July; 10(5): 509–516. PMCID: PMC2735332
6. Hewagama A, Richardson B. (2009). The genetics and epigenetics of autoimmune diseases. J Autoimmun. 2009 Aug; 33(1):3-11. Review. PMCID: PMC2819418
7. Basu D, Liu Y, Wu A, Yarlagadda S, Gorelik GJ, Kaplan MJ, Hewagama A, Hinderer RC, Strickland FM, Richardson BC.(2009). Stimulatory and inhibitory killer Ig-like receptor molecules are expressed and functional on lupus T cells. J Immunol. 2009 Sep 1; 183(5):3481-7. PMCID: PMC2730421
8. Strickland FM, Hewagama A, Lu Q, Wu A, Hinderer R, Webb R, Johnson K, Sawalha AH,
Delaney C, Yung R, Richardson BC. (2011). Environmental exposure, estrogen and two X
chromosomes are required for disease development in an epigenetic model of lupus.
J Autoimmun. 2012 May; 38(2-3):J135-43. Epub 2011 Dec 3. PMID: 22142890
9. Faith M. Strickland*, Anura Hewagama, Ailing Wu, Amr H. Sawalha, Colin Delaney, Mark F. Hoeltzel, Raymond Yung, Kent Johnson, Barbara Mickelson, and Bruce C. Richardson (2012),Diet Influences Expression of Autoimmune Associated Genes and Disease By Epigenetic Mechanisms in a Transgenic Lupus Model. Arthritis Rheum. 2013 Apr 10. doi: 10.1002/art.37967. [Epub ahead of print],PMID: 23576011
10. Anura Hewagama, Gabriela Gorelik, Dipak Patel, Amr Sawalha, Joan Merrill, R, Hal Scofield, W. Joseph McCune, Emily Somers, the Michigan Lupus Cohort, Punsisi Liyanarachchi, Faith Strickland and Bruce Richardson.(2013). Overexpression of X-linked genes in T cells from women with lupus. J Autoimmun. 2013 Feb 19., PMID: 23434382
11. Anura Hewagama, Role of X-chromosome encoded miRNAs in autoimmunity: suppressing the suppressor and female predisposition (2013), Accepted for publication (May 03, 2013), Rheumatology: Current Research (http://www.omicsonline.org/2161-1149/2161-1149-3-118.digital/2161-1149-3-118.html)
ABSTRACTS
1.Anura Hewagama, K.T.D. De Silva. (1988). Pharmacognostic Studies on Gmelina arborea. Sri Lanka Association for Advancement of Science (SLAAS) Annual Session.
2.Hedeel I. Guy, Anura Hewagama, David R. Evans. (1993). Identification of a region involved in interdomain signaling between the glutaminase and carbamoyl phosphate synthetase domain of CAD. 6th International symposium on purine and pyrimidine metabolism in man. Bloomington, IN.
3.Anura Hewagama, Hedeel I. Guy, David R. Evans. (1997). The mechanism of the glutaminase domain of mammalian carbamoyl phosphate synthetase. 9th International/6th European joint symposium on purine and pyrimidine metabolism in man, Gmunden, Austria.
4.Anura Hewagama, Hedeel I. Guy, David R. Evans. (1999). A spectrophotometric probe of the active site of glutaminase domain of mammalian CPSase. American Society for Biochemistry and Molecular Biology (ASBMB) annual meeting, San Francisco, CA.
5.Andrew Berkowski, Anura Hewagama, Hedeel I. Guy, David R. Evans. (2000). HPLC separation and quantitation of the nucleotides in measuring in vivo pyrimidine biosynthesis. Barbara Ann Karmanos Cancer Institute, Summer Fellowship Poster Presentation, Detroit, MI.
6.Moulin, A. Hewagama, I. Baburina, A. B. Shanafelt, D. Ringe, G. A. Petsko. (2005). One Key, many locks: recognition of drugs of abuse by antibodies. Protein Society Meeting, Boston, MA.
7.Anura Hewagama, Dipak Patel, Sushma Yarlagadda, Faith M. Strickland, Bruce C. Richardson (2007). Evaluation of gender differences in T cell gene expression by microarray analysis. American College of Rheumatology (ACR) Meeting, Boston, MA.
8.Anura Hewagama, Dipak Patel, Sushma Yarlagadda, Faith M. Strickland and Bruce C. Richardson. (2008). Evaluation of gender differences in methylation-sensitive immune gene expression in T cells by microarray. Experimental Biology, San Diego, CA. FASEB J. 2008 22:825.5.
9.Anura Hewagama, Dipak Patel and Bruce C. Richardson. (2009). Microarray based analysis of regulatory networks of methylation sensitive genes in T cells. The National Center for Integrative Biomedical Informatics (NCIBI) Fourth Annual Research Meeting, Ann Arbor, MI.
10.Anura Hewagama, Melanie Croos-Dabrera, Sushma Yarlagadda, Robert Hinderer, Dipak Patel and Bruce Richardson. (2010). X chromosome Signatures Associated with the MicroRNome of Human Peripheral Blood CD4+ T cells; Integrating the Epi-MicroRNome into the Molecular Basis of Human Lupus. 9th International Congress on Systemic Lupus Erythematosus, Vancouver, CA.
11.Haley Glatthorn, Punsisi Liyanarachchi, Anura Hewagama, Bruce Richardson (2011). Identifying DNA methylation patterns in human lupus. Symposium on Undergraduate Research Opportunity Program, University of Michigan, Ann Arbor, MI
12.Anura Hewagama, Punsisi Liyanaarachchi, Haley Glatthorn, Joshua Gross and Bruce Richardson (2011). Quantitation of CpG DNA Methylation using MBD affinity enrichment and Real-Time qPCR (MBD-qPCR). Symposium on Undergraduate Research Opportunity Program, University of Michigan, Ann Arbor, MI
13.Mathew Idichandy, Matt McGill, Punsisi Liyanaarachchi, Anura Hewagama and Bruce Richardson (2011). Altered Gene Expression in Defective Immune Regulatory Pathways of Human Lupus. Symposium on Undergraduate Research Opportunity Program, University of Michigan, Ann Arbor, MI
14.Anura Hewagama, Punsisi Liyanaarachchi, Joshua Gross, Haley Glatthorn, Mathew Idichandy and Bruce Richardson. (2011). ABERRANT DNA METHYLATION AND MICRORNA EXPRESSION IN HUMAN LUPUS, Department of Internal Medicine, 19th Annual Research Symposium, University of Michigan Health System.
RESERCH GRANTD
Grants Awarded
Sponsor MICHR
Grant Number U034555
Title C-CBL down regulation by microRNAs in CD4+ T cells, effect of miR-98 and the contribution to autoreactivity
PI Anura Hewagama, Ph.D.
Role PI
Total Project Period 2012 January- 2012 December
Total Direct Costs 50,000 yr
Grants Not Awarded
Sponsor: Health and Human Services Department, NIH
Grant Number: RFA-ES-11-002, R21
Title: DNA methylation changes associated with bisphenol A exposure: contributions to human lupus and potential dietary intervention
PI: Anura Hewagama, Ph.D.
Role: PI
Total Project Period: 2012-2015
Total Direct Costs: 100,000 yr
Sponsor: Alliance for Lupus Research
Grant Number: PAF00159
Title: MicroRNA miR-98 as a therapeutic target in lupus
PI: Anura Hewagama, Ph.D.
Role: PI
Total Project Period: July 2011-July 2013
Total Direct Costs: $250,000/year
4308 Hillside Dr.·Ann Arbor, MI 48105·Cell Phone: (734) 678-0724·Email: [email protected]
CITIZENSHIP: USA
CERTIFICATION: Michigan Secondary Teacher Certificate in Chemistry (DC).
EDUCATION
Mater of Arts in Teaching (2ry Chemistry): Eastern Michigan University, MI 06/2014-02/2015
- GPA: 3.6
Doctor of Philosophy, Biochemistry and Molecular Biology: Wayne State University, Detroit, MI
09/1992-08/1999
· Minor Subjects in Pharmaceutical Science and Computer Science
· Awards: Graduate Travel Award 1999, American Society for Biochemistry and Molecular Biology
Master of Science, Chemistry: Eastern Michigan University, Ypsilanti, MI 09/1990-06/1992
Post Baccalaureate Certificate, Molecular Laboratory Diagnostics: Michigan State University, Lansing, MI. GPA 4.0 06/2005-06/2006
Bachelor of Science, Biology/ Chemistry: University of Sri Jayewardenepura, Sri Lanka. 03/1982-05/1986
· Second Class Honors
TEACHING EXPERIENCE
Teacher – Chemistry: East English Village Preparatory Academy, Detroit Public Schools Community District, Detroit, MI 02/2016-Present
- High School Chemistry
Adjunct Instructor –Chemistry: Lansing Community College, Lansing, MI 08/2016-Present
- Undergraduate General Chemistry Lecture and Laboratory
Teaching Internship: Lincoln Consolidated High School/Eastern Michigan University, Ypsilanti, MI
01/2015-06/2015
Graduate Teaching Assistant: Department of Biochemistry and Molecular Biology, Wayne State
University, Detroit, MI 09/1993-05/1994
- BCH 701 General Biochemistry Lecture
Graduate Teaching Assistant: Department of Chemistry, Eastern Michigan University, Ypsilanti, MI
09/1990-05/1992
- Undergraduate Organic Chemistry, Biochemistry and General Chemistry Laboratory sessions
Instructor: Chemistry/Botany, University of Sri Jayewardenepura, Sri Lanka 06/1986-06/1989
PROFESSIONAL EXPERIENCE
Research Investigator: Rheumatology, University of Michigan, Ann Arbor 11/2009-12/ 2012
· Characterized molecular markers in Lupus disease development
· Received pilot funding from Michigan Institute for Clinical & Health Research (MICHR)
· Publications: PMID:22142890, PMID:23434382 and PMID:23576011
Research Lab Specialist Sr.: Rheumatology, University of Michigan, Ann Arbor 10/2006-11/2009
· Identified novel genes and biological pathways with potential contribution to lupus pathogenesis
· Publications: PMID: 19279650,PMID: 19349147 and PMID: 19675166
Research Lab Specialist Sr./Lab Manager: Human Genetics/Bioinformatics,
University of Michigan, Ann Arbor 07/2002-10/2006
· Regulation of gene expression and systems biology - Verification of microarray data using real time PCR, quantitative chromatin immunoprecipitation experiments to analyze NFkappaB regulated genes and pathways.
· Other responsibilities included training, technical assistance and supervision of new lab personnel and the laboratory management.
Post Doctoral Research Fellow: Roche Diagnostics, Indianapolis, IN. 08/2001-07/2002
Advisor: Armen B. Shanafelt, Ph.D.
· Antibody engineering – Structure guided design of antibodies for optimized affinity and specificity to antigens. Our research team solved the structures of the cocaine and morphine bound antibodies.
· Publications: PMID: 15885702 and PMID: 15019787
Postdoctoral Research Fellow: Department Biochemistry and Molecular Biology, Wayne State University, Detroit, MI. 12/1999-08/2001
Advisor: David Evans, Ph.D.
· Understanding the regulation of the mammalian pyrimidine biosynthetic pathway by kinase-mediated phosphorylation of the multifunctional protein CAD. Developed and improved HPLC assay conditions in monitoring the nucleotide levels during the regulation process.
Graduate Research Assistant: Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, MI 09/1994-06/1999
· Doctoral Thesis Research - Studied the mechanism of glutamine hydrolysis and the interdomain interactions of mammalian carbamoyl phosphate synthetase. By using steady state and pre-steady state kinetic studies, site-directed mutagenesis, and homology modeling approaches, we elucidated the molecular mechanism of glutamine hydrolysis and the regulation of the coupled synthesis of carbamoyl phosphate synthetase.
· Publications: PMID: 10497179,PMID: 9858783
Student Training in Research
· Participated in the University of Michigan Undergraduate Research Opportunity (UROP) program and supervised and trained 6 students 09/2009-12/2012
· I have also trained and supervised several work study students and high school students in laboratory research
Current Teaching Interests
· General and advanced Biochemistry
· Introductory level general, inorganic, and organic chemistry
· Application of Bioinformatics tools in Data mining and Biological Pathway Analysis
· Biotechnology and Life Science Research
COMMUNITY/ADMINSTRATIVE
MHealthy Wellness Champion (2010-2012)- University of Michigan
· Promoted and encouraged MHealthy programs and activities among colleagues and U of M Community.
Sports
· Cricket - Represented and captained the Ann Arbor Lions Cricket Club (2012-2015).
· Badminton - Represented and captained University of Sri Jayewardenepura at the inter-college badminton meets. I was awarded the University Badminton Colors (1982/83, 1983/84 and 1984/85 academic years). Currently a member of Ann Arbor Badminton Club.
Invited Reviews
Arthritis Research & Therapy – June 2012
Rheumatology Current Research- 2012 - 2014
Invited Editorial Board Member
OMICS Publishing Group- Rheumatology: Current Research-Open Access – 2012- 2014
Memberships in Professional Societies
January 2016 – Present Member, The National Society for Leadership and Success
March 2015 – Present Member, American Chemical Society
February 2015-March 2016 Member, Association for Education Communication and Technology
1996-2012 Member, American Society for Biochemistry and Molecular Biology
1996-2001 Member, Biophysical Society
PUBLICATIONS IN PEER-REVIEWED JOURNALS
1.Hewagama A, Guy HI, Chaparian M, Evans DR. (1998). The function of Glu338 in the catalytic triad of the carbamoyl phosphate synthetase amidotransferase domain. Biochim Biophys Acta. 1998 Nov 10; 1388(2):489-99. PMID: 9858783
2.Hewagama A, Guy HI, Vickrey JF, Evans DR. (1999). Functional linkage between the glutaminase and synthetase domains of carbamoyl-phosphate synthetase. Role of serine 44 in carbamoyl-phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD). J Biol Chem. 1999 Oct 1; 274(40):28240-5. PMID: 10497179
3.Pozharski E, Wilson MA, Hewagama A, Shanafelt AB, Petsko G, Ringe D. (2004). Anchoring a cationic ligand: the structure of the Fab fragment of the anti-morphine antibody 9B1 and its complex with morphine. J Mol Biol. 2004 Mar 26; 337(3):691-7. PMID: 15019787
4.Pozharski E, Moulin A, Hewagama A, Shanafelt AB, Petsko GA, Ringe D. (2005). Diversity in hapten recognition: structural study of an anti-cocaine antibody M82G2. J Mol Biol. 2005 Jun 10; 349(3):570-82. PMID: 1588502
5. Hewagama A, Patel D, Yarlagadda S, Strickland FM, Richardson BC. (2009). Stronger inflammatory/cytotoxic T cell response in women identified by microarray analysis. Genes Immun. 2009 July; 10(5): 509–516. PMCID: PMC2735332
6. Hewagama A, Richardson B. (2009). The genetics and epigenetics of autoimmune diseases. J Autoimmun. 2009 Aug; 33(1):3-11. Review. PMCID: PMC2819418
7. Basu D, Liu Y, Wu A, Yarlagadda S, Gorelik GJ, Kaplan MJ, Hewagama A, Hinderer RC, Strickland FM, Richardson BC.(2009). Stimulatory and inhibitory killer Ig-like receptor molecules are expressed and functional on lupus T cells. J Immunol. 2009 Sep 1; 183(5):3481-7. PMCID: PMC2730421
8. Strickland FM, Hewagama A, Lu Q, Wu A, Hinderer R, Webb R, Johnson K, Sawalha AH,
Delaney C, Yung R, Richardson BC. (2011). Environmental exposure, estrogen and two X
chromosomes are required for disease development in an epigenetic model of lupus.
J Autoimmun. 2012 May; 38(2-3):J135-43. Epub 2011 Dec 3. PMID: 22142890
9. Faith M. Strickland*, Anura Hewagama, Ailing Wu, Amr H. Sawalha, Colin Delaney, Mark F. Hoeltzel, Raymond Yung, Kent Johnson, Barbara Mickelson, and Bruce C. Richardson (2012),Diet Influences Expression of Autoimmune Associated Genes and Disease By Epigenetic Mechanisms in a Transgenic Lupus Model. Arthritis Rheum. 2013 Apr 10. doi: 10.1002/art.37967. [Epub ahead of print],PMID: 23576011
10. Anura Hewagama, Gabriela Gorelik, Dipak Patel, Amr Sawalha, Joan Merrill, R, Hal Scofield, W. Joseph McCune, Emily Somers, the Michigan Lupus Cohort, Punsisi Liyanarachchi, Faith Strickland and Bruce Richardson.(2013). Overexpression of X-linked genes in T cells from women with lupus. J Autoimmun. 2013 Feb 19., PMID: 23434382
11. Anura Hewagama, Role of X-chromosome encoded miRNAs in autoimmunity: suppressing the suppressor and female predisposition (2013), Accepted for publication (May 03, 2013), Rheumatology: Current Research (http://www.omicsonline.org/2161-1149/2161-1149-3-118.digital/2161-1149-3-118.html)
ABSTRACTS
1.Anura Hewagama, K.T.D. De Silva. (1988). Pharmacognostic Studies on Gmelina arborea. Sri Lanka Association for Advancement of Science (SLAAS) Annual Session.
2.Hedeel I. Guy, Anura Hewagama, David R. Evans. (1993). Identification of a region involved in interdomain signaling between the glutaminase and carbamoyl phosphate synthetase domain of CAD. 6th International symposium on purine and pyrimidine metabolism in man. Bloomington, IN.
3.Anura Hewagama, Hedeel I. Guy, David R. Evans. (1997). The mechanism of the glutaminase domain of mammalian carbamoyl phosphate synthetase. 9th International/6th European joint symposium on purine and pyrimidine metabolism in man, Gmunden, Austria.
4.Anura Hewagama, Hedeel I. Guy, David R. Evans. (1999). A spectrophotometric probe of the active site of glutaminase domain of mammalian CPSase. American Society for Biochemistry and Molecular Biology (ASBMB) annual meeting, San Francisco, CA.
5.Andrew Berkowski, Anura Hewagama, Hedeel I. Guy, David R. Evans. (2000). HPLC separation and quantitation of the nucleotides in measuring in vivo pyrimidine biosynthesis. Barbara Ann Karmanos Cancer Institute, Summer Fellowship Poster Presentation, Detroit, MI.
6.Moulin, A. Hewagama, I. Baburina, A. B. Shanafelt, D. Ringe, G. A. Petsko. (2005). One Key, many locks: recognition of drugs of abuse by antibodies. Protein Society Meeting, Boston, MA.
7.Anura Hewagama, Dipak Patel, Sushma Yarlagadda, Faith M. Strickland, Bruce C. Richardson (2007). Evaluation of gender differences in T cell gene expression by microarray analysis. American College of Rheumatology (ACR) Meeting, Boston, MA.
8.Anura Hewagama, Dipak Patel, Sushma Yarlagadda, Faith M. Strickland and Bruce C. Richardson. (2008). Evaluation of gender differences in methylation-sensitive immune gene expression in T cells by microarray. Experimental Biology, San Diego, CA. FASEB J. 2008 22:825.5.
9.Anura Hewagama, Dipak Patel and Bruce C. Richardson. (2009). Microarray based analysis of regulatory networks of methylation sensitive genes in T cells. The National Center for Integrative Biomedical Informatics (NCIBI) Fourth Annual Research Meeting, Ann Arbor, MI.
10.Anura Hewagama, Melanie Croos-Dabrera, Sushma Yarlagadda, Robert Hinderer, Dipak Patel and Bruce Richardson. (2010). X chromosome Signatures Associated with the MicroRNome of Human Peripheral Blood CD4+ T cells; Integrating the Epi-MicroRNome into the Molecular Basis of Human Lupus. 9th International Congress on Systemic Lupus Erythematosus, Vancouver, CA.
11.Haley Glatthorn, Punsisi Liyanarachchi, Anura Hewagama, Bruce Richardson (2011). Identifying DNA methylation patterns in human lupus. Symposium on Undergraduate Research Opportunity Program, University of Michigan, Ann Arbor, MI
12.Anura Hewagama, Punsisi Liyanaarachchi, Haley Glatthorn, Joshua Gross and Bruce Richardson (2011). Quantitation of CpG DNA Methylation using MBD affinity enrichment and Real-Time qPCR (MBD-qPCR). Symposium on Undergraduate Research Opportunity Program, University of Michigan, Ann Arbor, MI
13.Mathew Idichandy, Matt McGill, Punsisi Liyanaarachchi, Anura Hewagama and Bruce Richardson (2011). Altered Gene Expression in Defective Immune Regulatory Pathways of Human Lupus. Symposium on Undergraduate Research Opportunity Program, University of Michigan, Ann Arbor, MI
14.Anura Hewagama, Punsisi Liyanaarachchi, Joshua Gross, Haley Glatthorn, Mathew Idichandy and Bruce Richardson. (2011). ABERRANT DNA METHYLATION AND MICRORNA EXPRESSION IN HUMAN LUPUS, Department of Internal Medicine, 19th Annual Research Symposium, University of Michigan Health System.
- Anura Hewagama, Katarina Evans, Haley N. Glatthorn, Joshua Gross and Bruce Richardson. (2012). Overexpression of X-chromosome encoded protein coding and microRNA genes: female predisposition to lupus. Department of Internal Medicine, 20th Annual Research Symposium, University of Michigan Health System.
RESERCH GRANTD
Grants Awarded
Sponsor MICHR
Grant Number U034555
Title C-CBL down regulation by microRNAs in CD4+ T cells, effect of miR-98 and the contribution to autoreactivity
PI Anura Hewagama, Ph.D.
Role PI
Total Project Period 2012 January- 2012 December
Total Direct Costs 50,000 yr
Grants Not Awarded
Sponsor: Health and Human Services Department, NIH
Grant Number: RFA-ES-11-002, R21
Title: DNA methylation changes associated with bisphenol A exposure: contributions to human lupus and potential dietary intervention
PI: Anura Hewagama, Ph.D.
Role: PI
Total Project Period: 2012-2015
Total Direct Costs: 100,000 yr
Sponsor: Alliance for Lupus Research
Grant Number: PAF00159
Title: MicroRNA miR-98 as a therapeutic target in lupus
PI: Anura Hewagama, Ph.D.
Role: PI
Total Project Period: July 2011-July 2013
Total Direct Costs: $250,000/year